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Image Search Results
Journal: PLoS ONE
Article Title: Development of a High-Content Orthopoxvirus Infectivity and Neutralization Assays
doi: 10.1371/journal.pone.0138836
Figure Lengend Snippet: Bivariate scatter correlation plot between vaccinia virus infectivity measured by high-content analysis based infectivity assay (HC-IA) (Y-axis) and the plaque assay (X-axis) presented in log 10 scale. Potencies of thirteen stocks of vaccinia virus were measured in plaque assay and HC-IA. Results were analyzed using GraphPad Prizm software showing significant correlation between image-based and standard plaque counting assays (Pearson r—0.95, P value—0.0001, R 2 —0.9, slope 0.83). Dashed line represents the ideal linear curve with slope of 1, while solid line is an observed concordance curve.
Article Snippet: To evaluate the agreement between plaque assay and HC-IA we used
Techniques: Infection, High Content Screening, Plaque Assay, Software
Journal: PLoS ONE
Article Title: Development of a High-Content Orthopoxvirus Infectivity and Neutralization Assays
doi: 10.1371/journal.pone.0138836
Figure Lengend Snippet: New Orthopoxvirus neutralization assay (HC-OVNA), Y-axis, and GFP-expressing vaccinia virus (HCS-GFP), X-axis, presented in log 10 scale. Correlation between two assays was evaluated using 49 human serum samples from smallpox vaccine study (CDC IRB protocol 3349). Mean EC 50 values from neutralization curves from HC-OVNA and HCS-GFP assays were plotted against each other (log scale) and analyzed using GraphPad Prizm software, showing significant correlation between two assays (Pearson r—0.9, P value—0.0001, R2–0.8).
Article Snippet: To evaluate the agreement between plaque assay and HC-IA we used
Techniques: Neutralization, Expressing, Software